The gut bacterium and pathobiont Bacteroides vulgatus activates NF-κB in a human gut epithelial cell line in a strain and growth phase dependent manner.
Identifieur interne : 000210 ( Main/Exploration ); précédent : 000209; suivant : 000211The gut bacterium and pathobiont Bacteroides vulgatus activates NF-κB in a human gut epithelial cell line in a strain and growth phase dependent manner.
Auteurs : Páraic Cuív [Australie] ; Tomas De Wouters [France] ; Rabina Giri [Australie] ; Stanislas Mondot [France] ; Wendy J. Smith [Australie] ; Hervé M. Blottière [France] ; Jakob Begun [Australie] ; Mark Morrison [Australie]Source :
- Anaerobe [ 1095-8274 ] ; 2017.
Abstract
The gut microbiota is increasingly implicated in the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC) although the identity of the bacteria that underpin these diseases has remained elusive. The pathobiont Bacteroides vulgatus has been associated with both diseases although relatively little is known about how its growth and functional activity might drive the host inflammatory response. We identified an ATP Binding Cassette (ABC) export system and lipoprotein in B. vulgatus ATCC 8482 and B. vulgatus PC510 that displayed significant sequence similarity to an NF-κB immunomodulatory regulon previously identified on a CD-derived metagenomic fosmid clone. Interestingly, the ABC export system was specifically enriched in CD subjects suggesting that it may be important for colonization and persistence in the CD gut environment. Both B. vulgatus ATCC 8482 and PC510 activated NF-κB in a strain and growth phase specific manner in a HT-29/kb-seap-25 enterocyte like cell line. B. vulgatus ATCC 8482 also activated NF-κB in a Caco-2-NF-κBluc enterocyte like and an LS174T-NF-κBluc goblet cell like cell lines, and induced NF-κB-p65 subunit nuclear translocation and IL-6, IL-8, CXCL-10 and MCP-1 gene expression. Despite this, NF-κB activation was not coincident with maximal expression of the ABC exporter or lipoprotein in B. vulgatus PC510 suggesting that the regulon may be necessary but not sufficient for the immunomodulatory effects.
DOI: 10.1016/j.anaerobe.2017.06.002
PubMed: 28583864
Affiliations:
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Smith</name><affiliation wicri:level="1"><nlm:affiliation>CSIRO Preventative Health Flagship Research Program, Queensland, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>CSIRO Preventative Health Flagship Research Program, Queensland</wicri:regionArea><wicri:noRegion>Queensland</wicri:noRegion></affiliation></author><author><name sortKey="Blottiere, Herve M" sort="Blottiere, Herve M" uniqKey="Blottiere H" first="Hervé M" last="Blottière">Hervé M. Blottière</name><affiliation wicri:level="1"><nlm:affiliation>Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France; MGP MetaGenoPolis, INRA, Université Paris-Saclay, Jouy-en-Josas, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France; MGP MetaGenoPolis, INRA, Université Paris-Saclay, Jouy-en-Josas</wicri:regionArea><wicri:noRegion>Jouy-en-Josas</wicri:noRegion><wicri:noRegion>Jouy-en-Josas</wicri:noRegion></affiliation></author><author><name sortKey="Begun, Jakob" sort="Begun, Jakob" uniqKey="Begun J" first="Jakob" last="Begun">Jakob Begun</name><affiliation wicri:level="1"><nlm:affiliation>Mater Research Institute - The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Mater Research Institute - The University of Queensland, Translational Research Institute, Brisbane, QLD</wicri:regionArea><wicri:noRegion>QLD</wicri:noRegion></affiliation></author><author><name sortKey="Morrison, Mark" sort="Morrison, Mark" uniqKey="Morrison M" first="Mark" last="Morrison">Mark Morrison</name><affiliation wicri:level="1"><nlm:affiliation>The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia; CSIRO Preventative Health Flagship Research Program, Queensland, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia; CSIRO Preventative Health Flagship Research Program, Queensland</wicri:regionArea><wicri:noRegion>Queensland</wicri:noRegion></affiliation></author></analytic><series><title level="j">Anaerobe</title><idno type="eISSN">1095-8274</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The gut microbiota is increasingly implicated in the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC) although the identity of the bacteria that underpin these diseases has remained elusive. The pathobiont Bacteroides vulgatus has been associated with both diseases although relatively little is known about how its growth and functional activity might drive the host inflammatory response. We identified an ATP Binding Cassette (ABC) export system and lipoprotein in B. vulgatus ATCC 8482 and B. vulgatus PC510 that displayed significant sequence similarity to an NF-κB immunomodulatory regulon previously identified on a CD-derived metagenomic fosmid clone. Interestingly, the ABC export system was specifically enriched in CD subjects suggesting that it may be important for colonization and persistence in the CD gut environment. Both B. vulgatus ATCC 8482 and PC510 activated NF-κB in a strain and growth phase specific manner in a HT-29/kb-seap-25 enterocyte like cell line. B. vulgatus ATCC 8482 also activated NF-κB in a Caco-2-NF-κBluc enterocyte like and an LS174T-NF-κBluc goblet cell like cell lines, and induced NF-κB-p65 subunit nuclear translocation and IL-6, IL-8, CXCL-10 and MCP-1 gene expression. Despite this, NF-κB activation was not coincident with maximal expression of the ABC exporter or lipoprotein in B. vulgatus PC510 suggesting that the regulon may be necessary but not sufficient for the immunomodulatory effects.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country></list><tree><country name="Australie"><noRegion><name sortKey=" Cuiv, Paraic" sort=" Cuiv, Paraic" uniqKey=" Cuiv P" first="Páraic" last=" Cuív">Páraic Cuív</name></noRegion><name sortKey="Begun, Jakob" sort="Begun, Jakob" uniqKey="Begun J" first="Jakob" last="Begun">Jakob Begun</name><name sortKey="Giri, Rabina" sort="Giri, Rabina" uniqKey="Giri R" first="Rabina" last="Giri">Rabina Giri</name><name sortKey="Morrison, Mark" sort="Morrison, Mark" uniqKey="Morrison M" first="Mark" last="Morrison">Mark Morrison</name><name sortKey="Smith, Wendy J" sort="Smith, Wendy J" uniqKey="Smith W" first="Wendy J" last="Smith">Wendy J. Smith</name></country><country name="France"><noRegion><name sortKey="De Wouters, Tomas" sort="De Wouters, Tomas" uniqKey="De Wouters T" first="Tomas" last="De Wouters">Tomas De Wouters</name></noRegion><name sortKey="Blottiere, Herve M" sort="Blottiere, Herve M" uniqKey="Blottiere H" first="Hervé M" last="Blottière">Hervé M. Blottière</name><name sortKey="Mondot, Stanislas" sort="Mondot, Stanislas" uniqKey="Mondot S" first="Stanislas" last="Mondot">Stanislas Mondot</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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